肝脏抗氧化剂敏感性呼吸。乙醇、铁和线粒体解偶联的影响。
Hepatic antioxidant-sensitive respiration. Effect of ethanol, iron and mitochondrial uncoupling.
作者信息
Videla L A
出版信息
Biochem J. 1984 Nov 1;223(3):885-91. doi: 10.1042/bj2230885.
The addition of the antioxidants (+)-cyanidanol-3, butylated hydroxyanisole and ascorbate to the perfused rat liver resulted in a decrease in the rate of oxygen consumption. This basal antioxidant-sensitive respiration of 110-130nmol X min-1 X (g of liver)-1 represents 5-7% of total respiration. Increased antioxidant-sensitive respiratory rates are found after the infusion of increasing concentrations of ethanol (1.8-72.2mM) or iron (35.5-248.5 microM). This respiratory component exhibits a dependence on ethanol or iron concentration, with maximal rates of 200-255 and 330nmol X min-1 X (g of liver)-1 respectively. After the addition of 100 microM-2,4-dinitriphenol, an antioxidant-sensitive respiratory component of 230nmol X min-1 X (g of liver)-1 is found, which is not observed at lower concentrations of the uncoupler (5-50 microM). The lack of effect of the antioxidants used on mitochondrial respiration [the preceding paper, Videla, Villena, Donoso, Giulivi & Boveris (1984) Biochem. J. 223, 879-883] and on the glycolytic rate of the perfused liver suggests that the basal and chemically induced antioxidant-sensitive respiration observed are related to oxygen required for one-electron transfer reactions associated with the generation of active species of oxygen and lipid peroxidation in the liver cell.
向灌注的大鼠肝脏中添加抗氧化剂（+）-氰定醇-3、丁基羟基茴香醚和抗坏血酸盐会导致耗氧率降低。这种基础的抗氧化剂敏感呼吸速率为110 - 130nmol·min⁻¹·（g肝脏）⁻¹，占总呼吸的5 - 7%。在输注浓度不断增加的乙醇（1.8 - 72.2mM）或铁（35.5 - 248.5μM）后，发现抗氧化剂敏感呼吸速率增加。这种呼吸成分表现出对乙醇或铁浓度的依赖性，最大速率分别为200 - 255和330nmol·min⁻¹·（g肝脏）⁻¹。添加100μM - 2,4 -二硝基苯酚后，发现有一个230nmol·min⁻¹·（g肝脏）⁻¹的抗氧化剂敏感呼吸成分，在较低浓度的解偶联剂（5 - 50μM）下未观察到。所使用的抗氧化剂对线粒体呼吸[前文，维德拉、维列纳、多诺索、朱利维 & 博韦里斯（1984年）《生物化学杂志》223卷，879 - 883页]以及对灌注肝脏的糖酵解速率没有影响，这表明所观察到的基础和化学诱导的抗氧化剂敏感呼吸与肝细胞中与活性氧和脂质过氧化生成相关的单电子转移反应所需的氧气有关。
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