Saggerson E D, Bird M I, Carpenter C A, Winter K A, Wright J J
Biochem J. 1984 Nov 15;224(1):201-6. doi: 10.1042/bj2240201.
Starvation (24h) increased the maximum activity of carnitine palmitoyltransferase 1 in rat liver and increased the concentration of malonyl-CoA required to cause 50% inhibition of the enzyme (I50). Re-feeding (24h) with a standard cube diet or a high-carbohydrate diet reversed both of these changes, whereas re-feeding with a high-fat diet did not. Administration of cycloheximide (200 micrograms/100 g body wt.) blocked the increases in carnitine palmitoyltransferase 1 activity and I50 on starvation. It is suggested that increase in carnitine palmitoyltransferase 1 activity in starvation may involve synthesis of new enzyme.
饥饿(24小时)可提高大鼠肝脏中肉碱棕榈酰转移酶1的最大活性,并增加导致该酶50%抑制率(I50)所需的丙二酰辅酶A浓度。用标准颗粒饲料或高碳水化合物饲料再喂养(24小时)可逆转这两种变化,而用高脂肪饲料再喂养则不能。给予环己酰亚胺(200微克/100克体重)可阻断饥饿时肉碱棕榈酰转移酶1活性和I50的增加。提示饥饿时肉碱棕榈酰转移酶1活性增加可能涉及新酶的合成。