pH对丙二酰辅酶A抑制肉碱棕榈酰转移酶I的影响。
Effect of pH on malonyl-CoA inhibition of carnitine palmitoyltransferase I.
作者信息
Stephens T W, Cook G A, Harris R A
出版信息
Biochem J. 1983 May 15;212(2):521-4. doi: 10.1042/bj2120521.
Abstract
Malonyl-CoA inhibition of carnitine palmitoyltransferase I was found to be very pH-dependent. Malonyl-CoA concentrations causing 50% inhibition (I50) at pH 6.0, 6.5, 7.0, 7.5 and 8.0 were 0.04, 1, 9, 40 and 200 microM respectively. It is suggested that a lowering of intracellular pH, such as might occur in ketoacidosis, may attenuate hepatic fatty acid oxidation by increasing malonyl-CoA sensitivity of carnitine palmitoyltransferase I.
摘要
丙二酰辅酶A对肉碱棕榈酰转移酶I的抑制作用被发现具有很强的pH依赖性。在pH 6.0、6.5、7.0、7.5和8.0时，引起50%抑制作用（I50）的丙二酰辅酶A浓度分别为0.04、1、9、40和200微摩尔。有人提出，细胞内pH降低，比如在酮症酸中毒时可能发生的情况，可能会通过增加肉碱棕榈酰转移酶I对丙二酰辅酶A的敏感性来减弱肝脏脂肪酸氧化。
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本文引用的文献
1
PALMITYL-COA:CARNITINE PALMITYLTRANSFERASE. PURIFICATION FROM CALF-LIVER MITOCHONDRIA AND SOME PROPERTIES OF THE ENZYME.棕榈酰辅酶A：肉碱棕榈酰转移酶。从小牛肝脏线粒体中纯化及该酶的一些性质
Biochim Biophys Acta. 1964 Jul 8;89:95-108.
2
CARNITINE IN INTERMEDIARY METABOLISM. THE BIOSYNTHESIS OF PALMITYLCARNITINE BY CELL SUBFRACTIONS.肉碱在中间代谢中的作用。细胞亚组分合成棕榈酰肉碱的过程。
J Biol Chem. 1963 Aug;238:2774-9.
3
Hyperpolarization of muscle by insulin in a glucose-free environment.在无葡萄糖环境中胰岛素引起的肌肉超极化。
Am J Physiol. 1959 Sep;197:524-6. doi: 10.1152/ajplegacy.1959.197.3.524.
4
Determination of the mitochondrial protonmotive force in isolated hepatocytes.分离的肝细胞中线粒体质子动力的测定。
J Biol Chem. 1980 Feb 25;255(4):1458-64.
5
Importance of experimental conditions in evaluating the malonyl-CoA sensitivity of liver carnitine acyltransferase. Studies with fed and starved rats.实验条件在评估肝脏肉碱酰基转移酶丙二酸单酰辅酶A敏感性中的重要性。对喂食和饥饿大鼠的研究。
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6
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Biochim Biophys Acta. 1981 Sep 24;665(3):628-31. doi: 10.1016/0005-2760(81)90282-4.
7
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