一种用于分离脂肪细胞的改良灌流培养系统。通过灌流研究中间代谢。
A modified perifused incubation system for isolated fat-cells. Investigation of intermediary metabolism by perifusion.
作者信息
Harper R D
出版信息
Biochem J. 1984 Nov 15;224(1):235-9. doi: 10.1042/bj2240235.
Abstract
Perifused fat-cells showed similar values for acylglycerol glycerol synthesis from glucose with insulin and for the effects of added palmitate to those in normal incubations and those reported in the literature. Fatty acid synthesis was lower in perifused cells compared with normal incubations, and there was a net release of fatty acids only with the perifused fat-cells. Hence fluxes of metabolites were different in the two incubation systems, and the perifusion system enables the investigation of the flux of metabolites under conditions which may more closely resemble those in vivo.
摘要
经灌流的脂肪细胞在由葡萄糖通过胰岛素合成酰基甘油甘油方面，以及在添加棕榈酸酯的作用方面，与正常孵育中的细胞及文献报道的情况显示出相似的值。与正常孵育相比，经灌流的细胞中脂肪酸合成较低，并且仅在经灌流的脂肪细胞中存在脂肪酸的净释放。因此，两种孵育系统中代谢物的通量不同，并且灌流系统能够在可能更接近体内情况的条件下研究代谢物的通量。
相似文献
1
A modified perifused incubation system for isolated fat-cells. Investigation of intermediary metabolism by perifusion.一种用于分离脂肪细胞的改良灌流培养系统。通过灌流研究中间代谢。
Biochem J. 1984 Nov 15;224(1):235-9. doi: 10.1042/bj2240235.
2
The response of adipocyte glucose metabolism and fatty acid release to adenosine deaminase, insulin and perifusion. Investigation of intermediary metabolism by perifusion.脂肪细胞葡萄糖代谢及脂肪酸释放对腺苷脱氨酶、胰岛素和灌流的反应。通过灌流研究中间代谢。
Biochem J. 1988 May 1;251(3):733-7. doi: 10.1042/bj2510733.
3
The regulation of glyceride synthesis in isolated white-fat cells. The effects of palmitate and lipolytic agents.分离的白色脂肪细胞中甘油酯合成的调节。棕榈酸盐和脂解剂的作用。
Biochem J. 1972 Aug;128(5):1057-67. doi: 10.1042/bj1281057.
4
The regulation of glyceride synthesis in isolated white-fat cells. The effects of acetate, pyruvate, lactate, palmitate, electron-acceptors, uncoupling agents and oligomycin.分离的白色脂肪细胞中甘油酯合成的调节。乙酸盐、丙酮酸、乳酸盐、棕榈酸盐、电子受体、解偶联剂和寡霉素的作用。
Biochem J. 1972 Aug;128(5):1069-78. doi: 10.1042/bj1281069.
5
Regulation of fatty acid synthesis and esterification in rat epididymal adipose tissue: effects of insulin, palmitate and 2-bromopalmitate [proceedings].
Biochem Soc Trans. 1977;5(5):1288-91. doi: 10.1042/bst0051288.
6
The regulation of glyceride synthesis from pyruvate in isolated fat cells. The effects of palmitate and alteration of dietary status.离体脂肪细胞中丙酮酸合成甘油酯的调节。棕榈酸酯的作用及饮食状态的改变。
Eur J Biochem. 1971 Nov 11;23(1):109-17. doi: 10.1111/j.1432-1033.1971.tb01597.x.
7
Lipogenesis in rat brown adipocytes. Effects of insulin and noradrenaline, contributions from glucose and lactate as precursors and comparisons with white adipocytes.大鼠棕色脂肪细胞中的脂肪生成。胰岛素和去甲肾上腺素的作用、葡萄糖和乳酸作为前体的贡献以及与白色脂肪细胞的比较。
Biochem J. 1988 May 1;251(3):701-9. doi: 10.1042/bj2510701.
8
Insulin-like stimulation of glucose transport in isolated adipocytes by fatty acids.脂肪酸对分离的脂肪细胞中葡萄糖转运的胰岛素样刺激作用。
Biochem Biophys Res Commun. 1985 May 16;128(3):1358-63. doi: 10.1016/0006-291x(85)91090-3.
9
The regulation of triglyceride synthesis and fatty acid synthesis in rat epididymal adipose tissue.大鼠附睾脂肪组织中甘油三酯合成与脂肪酸合成的调节
Biochem J. 1970 Sep;119(2):193-219. doi: 10.1042/bj1190193.
10
Chronic perifusion of rat islets with palmitate suppresses glucose-stimulated insulin release.用棕榈酸对大鼠胰岛进行慢性灌注会抑制葡萄糖刺激的胰岛素释放。
Endocrinology. 1993 Jul;133(1):208-14. doi: 10.1210/endo.133.1.8319569.
引用本文的文献
1
The response of adipocyte glucose metabolism and fatty acid release to adenosine deaminase, insulin and perifusion. Investigation of intermediary metabolism by perifusion.脂肪细胞葡萄糖代谢及脂肪酸释放对腺苷脱氨酶、胰岛素和灌流的反应。通过灌流研究中间代谢。
Biochem J. 1988 May 1;251(3):733-7. doi: 10.1042/bj2510733.
本文引用的文献
1
Feeding and breeding of laboratory animals; a complete cubed diet for mice and rats.实验动物的饲养与繁殖；小鼠和大鼠的全价颗粒饲料。
J Hyg (Lond). 1949 Jun;47(2):202-8. doi: 10.1017/s0022172400014479.
2
ON THE INTERPRETATION OF STUDIES MEASURING UPTAKE AND ESTERIFICATION OF (1-14-C)PALMITIC ACID BY RAT ADIPOSE TISSUE IN VITRO.关于大鼠脂肪组织体外对（1-¹⁴C）棕榈酸摄取和酯化的研究解读
Biochim Biophys Acta. 1964 Apr 20;84:154-66. doi: 10.1016/0926-6542(64)90072-1.
3
METABOLISM OF ISOLATED FAT CELLS. I. EFFECTS OF HORMONES ON GLUCOSE METABOLISM AND LIPOLYSIS.分离脂肪细胞的代谢。I. 激素对葡萄糖代谢和脂肪分解的影响。
J Biol Chem. 1964 Feb;239:375-80.
4
EFFECTS OF PALMITATE ON THE METABOLISM OF LEUKOCYTES FROM GUINEA PIG EXUDATE.棕榈酸盐对豚鼠渗出液中白细胞代谢的影响。
J Lipid Res. 1963 Jan;4:39-45.
5
PERFUSION OF RAT ADIPOSE TISSUE.大鼠脂肪组织灌注
Am J Physiol. 1963 Aug;205:405-12. doi: 10.1152/ajplegacy.1963.205.2.405.
6
A rapid enzymatic assay for glycerol.一种用于甘油的快速酶促测定法。
Nature. 1962 Dec 8;196:987-8. doi: 10.1038/196987a0.
7
The fatty acid pool in adipose tissue.
J Biol Chem. 1961 Dec;236:3121-4.
8
Influence of blood flow on fatty acid mobilization form lipolytically active adipose tissue.血流对脂解活性脂肪组织中脂肪酸动员的影响。
Pflugers Arch. 1981 May;390(2):169-74. doi: 10.1007/BF00590202.
9
Subcutaneous adipose tissue blood flow and triacylglycerol-mobilization during prolonged exercise in dogs.犬长时间运动期间的皮下脂肪组织血流与三酰甘油动员
Pflugers Arch. 1982 Jan;392(3):230-4. doi: 10.1007/BF00584301.
10
Alternative substrates for triacylglycerol synthesis in isolated adipocytes of different size from the rat.来自大鼠不同大小分离脂肪细胞中三酰甘油合成的替代底物。
Biochem J. 1981 Feb 15;194(2):377-84. doi: 10.1042/bj1940377.
Zotero 插件