一种用于分离脂肪细胞的改良灌流培养系统。通过灌流研究中间代谢。
A modified perifused incubation system for isolated fat-cells. Investigation of intermediary metabolism by perifusion.
作者信息
Harper R D
出版信息
Biochem J. 1984 Nov 15;224(1):235-9. doi: 10.1042/bj2240235.
Abstract
Perifused fat-cells showed similar values for acylglycerol glycerol synthesis from glucose with insulin and for the effects of added palmitate to those in normal incubations and those reported in the literature. Fatty acid synthesis was lower in perifused cells compared with normal incubations, and there was a net release of fatty acids only with the perifused fat-cells. Hence fluxes of metabolites were different in the two incubation systems, and the perifusion system enables the investigation of the flux of metabolites under conditions which may more closely resemble those in vivo.
摘要
经灌流的脂肪细胞在由葡萄糖通过胰岛素合成酰基甘油甘油方面，以及在添加棕榈酸酯的作用方面，与正常孵育中的细胞及文献报道的情况显示出相似的值。与正常孵育相比，经灌流的细胞中脂肪酸合成较低，并且仅在经灌流的脂肪细胞中存在脂肪酸的净释放。因此，两种孵育系统中代谢物的通量不同，并且灌流系统能够在可能更接近体内情况的条件下研究代谢物的通量。
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引用本文的文献
1
The response of adipocyte glucose metabolism and fatty acid release to adenosine deaminase, insulin and perifusion. Investigation of intermediary metabolism by perifusion.
Biochem J. 1988 May 1;251(3):733-7. doi: 10.1042/bj2510733.
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