Bolard J, Cheron M, Mazerski J
Biochem Pharmacol. 1984 Nov 15;33(22):3675-80. doi: 10.1016/0006-2952(84)90156-4.
The interactions of unilamellar lipid vesicles with vacidin A, an aromatic heptaene antibiotic, and with amphotericin B, a nonaromatic heptaene antibiotic were compared. Uptake of both antibiotics, monitored by circular dichroism, was found to be faster with small vesicles than with large ones. By combining permeability measurements (Gary-Bobo and Cybulska, J. Antibiotics 35, 1068 (1982)) and circular dichroism spectra, we found that for vacidin A, the same permeability inducing species is formed regardless of vesicles size. However, at a given concentration of antibiotic, less of the permeability inducing species is formed in the presence of small vesicles than in the presence of large vesicles. This may account for the differences between small and large vesicles in antibiotics-induced permeability. For amphotericin B, the permeability inducing species formed in the presence of small vesicles differs from that formed in the presence of large vesicles.
比较了单层脂质囊泡与芳香族七烯抗生素瓦西丁A以及非芳香族七烯抗生素两性霉素B的相互作用。通过圆二色性监测发现，两种抗生素在小囊泡中的摄取速度比在大囊泡中更快。通过结合渗透率测量（Gary - Bobo和Cybulska，《抗生素杂志》35，1068（1982））和圆二色性光谱，我们发现对于瓦西丁A，无论囊泡大小，都会形成相同的诱导渗透率的物种。然而，在给定浓度的抗生素下，小囊泡存在时形成的诱导渗透率的物种比大囊泡存在时少。这可能解释了小囊泡和大囊泡在抗生素诱导的渗透率方面存在的差异。对于两性霉素B，小囊泡存在时形成的诱导渗透率的物种与大囊泡存在时形成的不同。
