Ray F A, Bartholdi M F, Kraemer P M, Cram L S
Cancer Genet Cytogenet. 1986 Mar 1;21(1):35-51. doi: 10.1016/0165-4608(86)90199-8.
Spontaneous neoplastic progression in cultured Chinese hamster cells was studied at the earliest stage possible. Eighteen independent newly immortalized cell populations (from six individual Chinese hamsters) were characterized for karyotype instability. Colonies were selected from initial sparse platings of adult or fetal cells and were expanded for study. The chromosomes from these newly established cell lines were studied using a combination of G-banding and flow karyotype analysis. At a slightly later passage, the 18 cell lines were tested for tumorigenicity in nude mice. Frequent recurring chromosome changes were observed in the karyotypes. The most frequent changes were either total or partial trisomy of chromosome #3 (83%) and trisomy of chromosome #5 (61%). Only 4 of 18 clones (22%) were tumorigenic at the time of testing, and these had long latent periods. The presence of recurrent chromosome changes did not obligate these cell lines to become tumorigenic, but the karyotype instability appeared to be an indicator of the ongoing process of neoplasia.
在尽可能早的阶段对培养的中国仓鼠细胞中的自发肿瘤进展进行了研究。对来自六只中国仓鼠的18个独立的新永生化细胞群体进行了核型不稳定性特征分析。从成年或胎儿细胞的初始稀疏铺板中挑选克隆并进行扩增以供研究。使用G显带和流式核型分析相结合的方法研究了这些新建立的细胞系的染色体。在稍晚传代时，对这18个细胞系进行了裸鼠致瘤性测试。在核型中观察到频繁出现的染色体变化。最常见的变化是3号染色体的全部或部分三体（83%）和5号染色体的三体（61%）。在测试时，18个克隆中只有4个（22%）具有致瘤性，并且这些克隆具有较长的潜伏期。反复出现的染色体变化并不一定会使这些细胞系具有致瘤性，但核型不稳定性似乎是肿瘤形成过程的一个指标。
