Tarsio J F, Shapiro B L
Enzyme. 1984;32(3):188-92. doi: 10.1159/000469474.
Cibacron Blue F3GA, the chromophore of blue dextran, was tested at 4-16 mumol/l for possible inhibition of NADH dehydrogenase activity when added to mitochondrial preparations from cultured human skin fibroblasts. The free dye was shown to be a competitive inhibitor for NADH in the oxidation of NADH catalyzed by the mitochondrial enzyme. The Ki (5.8 mumol/l) for Cibacron Blue F3GA was considerably lower than the Michaelis constant (Km) found for NADH substrate (13.2-16.1 mumol/l), indicating a strong binding of the dye to the substrate-binding site of the enzyme. This is the first report of the competitive inhibition by Cibacron Blue F3GA of mitochondrial NADH in any species.
蓝色葡聚糖的发色团汽巴克隆蓝F3GA，在添加到培养的人皮肤成纤维细胞的线粒体制剂中时，以4 - 16微摩尔/升的浓度进行测试，以考察其对NADH脱氢酶活性的可能抑制作用。游离染料被证明是线粒体酶催化的NADH氧化中NADH的竞争性抑制剂。汽巴克隆蓝F3GA的Ki（5.8微摩尔/升）远低于NADH底物的米氏常数（Km）（13.2 - 16.1微摩尔/升），表明该染料与酶的底物结合位点有很强的结合力。这是关于汽巴克隆蓝F3GA对任何物种线粒体NADH竞争性抑制作用的首次报道。
