还原型氟哌啶醇代谢物对血液中氟哌啶醇放射免疫测定和放射受体测定可能产生的干扰。

Possible interference by the reduced haloperidol metabolite with the radioimmunoassay and radioreceptor assay of blood haloperidol.

作者信息

Korpi E R, Ko G N, Phelps B H, Wyatt R J

出版信息

J Clin Psychopharmacol. 1984 Dec;4(6):332-5.

PMID:6512001

Abstract

Serum or plasma samples from haloperidol-treated patients were analyzed by high performance liquid chromatography (HPLC) with electrochemical detection, radioimmunoassay (RIA), and radioreceptor assay (RRA). The HPLC assay allows simultaneous quantitation of the reduced alcohol metabolite of haloperidol. The HPLC and RIA haloperidol results correlated fairly well (r = 0.63), while the HPLC reduced haloperidol and the RIA haloperidol had a weak correlation (r = 0.28). The RRA haloperidol results had a fair correlation with those of the HPLC (r = 0.55), but their correlation with the HPLC reduced haloperidol was almost as good (r = 0.52). The RIA tended to give lower and the RRA higher apparent haloperidol concentrations than the HPLC assay. The results indicate that the reduced haloperidol does not interfere with the RIA procedure used in this study, but it may partially account for higher concentrations obtained with the RRA.

摘要

采用高效液相色谱法（HPLC）结合电化学检测、放射免疫分析法（RIA）和放射受体分析法（RRA）对接受氟哌啶醇治疗患者的血清或血浆样本进行分析。HPLC 分析法可同时对氟哌啶醇的还原型酒精代谢物进行定量。HPLC 和 RIA 法检测氟哌啶醇的结果相关性较好（r = 0.63），而 HPLC 法检测的还原型氟哌啶醇与 RIA 法检测的氟哌啶醇相关性较弱（r = 0.28）。RRA 法检测氟哌啶醇的结果与 HPLC 法的结果相关性较好（r = 0.55），但与 HPLC 法检测的还原型氟哌啶醇的相关性几乎相同（r = 0.52）。与 HPLC 分析法相比，RIA 法测得的氟哌啶醇表观浓度往往较低，而 RRA 法测得的较高。结果表明，还原型氟哌啶醇不会干扰本研究中使用的 RIA 程序，但可能是 RRA 法测得较高浓度的部分原因。