用环己酰亚胺处理诱导小鼠成纤维细胞中免疫球蛋白基因表达。
Induction of immunoglobulin gene expression in mouse fibroblasts by cycloheximide treatment.
作者信息
Ishihara T, Kudo A, Watanabe T
出版信息
J Exp Med. 1984 Dec 1;160(6):1937-42. doi: 10.1084/jem.160.6.1937.
Abstract
A complete set of a rearranged human gamma 1-heavy chain gene, HIG1, was cloned from human plasma cell leukemia line, ARH-77, and transferred into mouse cells. It was strongly expressed in mouse myeloma cells but not in mouse L cells, indicating that immunoglobulin gene expression is not species-specific but cell-specific. However, a remarkable production of human gamma 1 chain was induced in mouse L cells containing HIG1 gene when the cells were treated with cycloheximide for a short period. The role of a labile repressor molecule in the expression of the immunoglobulin gene is proposed.
摘要
从人浆细胞白血病细胞系ARH - 77中克隆出一套完整的重排人γ1重链基因HIG1，并将其转入小鼠细胞。它在小鼠骨髓瘤细胞中强烈表达，但在小鼠L细胞中不表达，这表明免疫球蛋白基因的表达不是物种特异性的，而是细胞特异性的。然而，当用环己酰亚胺短期处理含有HIG1基因的小鼠L细胞时，可诱导其产生大量人γ1链。本文提出了不稳定阻遏分子在免疫球蛋白基因表达中的作用。
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