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具有人恒定区和针对癌相关抗原17-1A的小鼠可变区的嵌合抗体。

Chimeric antibody with human constant regions and mouse variable regions directed against carcinoma-associated antigen 17-1A.

作者信息

Sun L K, Curtis P, Rakowicz-Szulczynska E, Ghrayeb J, Chang N, Morrison S L, Koprowski H

出版信息

Proc Natl Acad Sci U S A. 1987 Jan;84(1):214-8. doi: 10.1073/pnas.84.1.214.

DOI:10.1073/pnas.84.1.214
PMID:3025856
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC304173/
Abstract

We have cloned the genomic DNA fragments encoding the heavy and light chain variable regions of monoclonal antibody 17-1A, and we have inserted them into mammalian expression vectors containing genomic DNA segments encoding human gamma 3 and kappa constant regions. The transfer of these expression vectors containing mouse-human chimeric immunoglobulin genes into Sp2/0 mouse myeloma cells resulted in the production of functional IgG that retained the specific binding to the surface antigen 17-1A expressed on colorectal carcinoma cells.

摘要

我们克隆了编码单克隆抗体17-1A重链和轻链可变区的基因组DNA片段，并将它们插入到含有编码人γ3和κ恒定区基因组DNA片段的哺乳动物表达载体中。将这些含有小鼠-人嵌合免疫球蛋白基因的表达载体转入Sp2/0小鼠骨髓瘤细胞后，产生了功能性IgG，该IgG保留了与结肠癌细胞表面表达的抗原17-1A的特异性结合。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e220/304173/8ba8ce525420/pnas00266-0233-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e220/304173/14fdfc326456/pnas00266-0232-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e220/304173/8ba8ce525420/pnas00266-0233-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e220/304173/14fdfc326456/pnas00266-0232-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e220/304173/8ba8ce525420/pnas00266-0233-a.jpg

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本文引用的文献

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Cancer Res. 1980 Mar;40(3):717-21.
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Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase.筛选表达编码黄嘌呤 - 鸟嘌呤磷酸核糖转移酶的大肠杆菌基因的动物细胞。
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Effect of gelonin immunoconjugate with monoclonal antibody MSN-1 to endometrial adenocarcinoma on antigen-producing tumor cells in vivo.与单克隆抗体MSN-1偶联的相思子毒素免疫偶联物对子宫内膜腺癌体内产抗原肿瘤细胞的作用。
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