Hayashi Y, Noda K, Shirasaka A, Nogami S, Nakamura M
Jpn J Exp Med. 1984 Aug;54(4):177-81.
The vaccination with radiation-attenuated infective larvae of Brugia malayi and B. pahangi was attempted and evaluated in BALB/c mice. Two weeks after intraperitoneal infection with 100 3rd stage larvae, the worms of both species of Brugia were recovered in the peritoneal cavity of BALB/c mice. The average recovery was more than 20% in both Brugia infections. Groups of 10 mice were vaccinated subsequently three times with 100 3rd stage larvae of B. malayi or B. pahangi attenuated by 20 krad gamma irradiation and challenged with the homologous species. Vaccinated mice showed a 95.5% reduction in recovered worms in the challenge infection with B. malayi as compared to the infection in non-vaccinated controls, and a 93.8% reduction in the B. pahangi group.
尝试用经辐射减毒的马来布鲁线虫和彭亨布鲁线虫感染性幼虫对BALB/c小鼠进行疫苗接种并进行评估。腹腔注射100条第三期幼虫两周后，在BALB/c小鼠的腹腔中回收了两种布鲁线虫。两种布鲁线虫感染的平均回收率均超过20%。随后，将10只小鼠分为一组，用经20千拉德伽马射线辐照减毒的100条马来布鲁线虫或彭亨布鲁线虫第三期幼虫进行三次疫苗接种，并用同源物种进行攻击。与未接种疫苗的对照组相比，接种疫苗的小鼠在马来布鲁线虫攻击感染中的回收虫数减少了95.5%，在彭亨布鲁线虫组中减少了93.8%。
