使用亚磷酰胺法自动合成寡脱氧核苷酸和混合寡脱氧核苷酸。
Automatic synthesis of oligodeoxynucleotides and mixed oligodeoxynucleotides using the phosphoamidite method.
作者信息
Josephson S, Lagerholm E, Palm G
出版信息
Acta Chem Scand B. 1984;38(7):539-45. doi: 10.3891/acta.chem.scand.38b-0539.
Abstract
A simplified procedure for making and using the deoxynucleoside phosphoamidites (1 a-d) in the automated synthesis of oligodeoxynucleotides has been developed. The reagents prepared show good stability in solution. The reactivity of the reagents was found to be directly proportional to their concentrations, which makes it possible to synthesize mixed oligomers in desired ratios. Oligomers up to 45-mer have been synthesized using a coupling cycle of twenty min.
摘要
已开发出一种在寡脱氧核苷酸自动合成中制备和使用脱氧核苷亚磷酰胺（1a - d）的简化方法。所制备的试剂在溶液中表现出良好的稳定性。发现试剂的反应性与其浓度成正比，这使得以所需比例合成混合寡聚物成为可能。使用20分钟的偶联循环已合成了长达45聚体的寡聚物。
Zotero 插件