Ike Y, Ikuta S, Sato M, Huang T, Itakura K
Nucleic Acids Res. 1983 Jan 25;11(2):477-88. doi: 10.1093/nar/11.2.477.
A solid phase method for the simultaneous synthesis of mixed oligonucleotides using a phosphotriester approach has been developed. For this synthesis, a mixture of mono or dimeric coupling units is used, and a slight difference in the reactivity of those units is found. However, this difference does not hamper the simultaneous, mixed oligonucleotide synthesis, and the sequence analysis of a product demonstrates the existence of all desired sequences in the final mixture.
已开发出一种使用磷酸三酯法同时合成混合寡核苷酸的固相方法。对于这种合成,使用了单聚体或二聚体偶联单元的混合物,并且发现这些单元的反应性存在轻微差异。然而,这种差异并不妨碍同时进行混合寡核苷酸的合成,并且产物的序列分析表明最终混合物中存在所有所需序列。