Fiore D, Auger F A, Drusano G L, Dandu V R, Lesko L J
Antimicrob Agents Chemother. 1984 Nov;26(5):775-7. doi: 10.1128/AAC.26.5.775.
A rapid, sensitive, and specific method of analysis for mezlocillin in serum and urine by high-pressure liquid chromatography is described. A solid-phase extraction column was used to remove interfering substances from samples before chromatography. Quantitation included the use of an internal standard, nafcillin. Mezlocillin was chromatographed with a phosphate buffer-acetonitrile (73:27) mobile phase and a C-18 reverse-phase column and detected at a wavelength of 220 nm. The assay had a sensitivity of 1.6 micrograms/ml and a linearity of up to 600 micrograms/ml and 16 mg/ml in serum and urine, respectively, with only 0.1 ml of sample. The interday and intraday coefficients of variation for replicate analyses of spiked serum and urine specimens were less than 6.5%.
描述了一种通过高压液相色谱法快速、灵敏且特异分析血清和尿液中美洛西林的方法。在色谱分析前，使用固相萃取柱去除样品中的干扰物质。定量分析采用内标物萘夫西林。美洛西林采用磷酸盐缓冲液 - 乙腈（73:27）流动相和C - 18反相柱进行色谱分离，并在220 nm波长处检测。该测定法的灵敏度为1.6微克/毫升，血清和尿液中的线性范围分别高达600微克/毫升和16毫克/毫升，仅需0.1毫升样品。加标血清和尿液标本重复分析的日间和日内变异系数均小于6.5%。
