Aoki N, Tateno K, Sakata Y
Biochem Genet. 1984 Oct;22(9-10):871-81. doi: 10.1007/BF00499478.
Frequency distributions of various plasminogen phenotypes in Japanese and American white populations were studied using electrofocusing in polyacrylamide gels followed by zymography and immunofixation. Using a synthetic substrate, tosyl-lysine-alpha-naphthyl ester, for zymography allowed zymography and immunofixation to be performed sequentially on the same gel plate. By this method, a nonfunctional abnormal plasminogen variant, plasminogen Tochigi, was readily detected in both plasma and serum. The gene frequency of this abnormal variant in a Japanese population was 0.018, whereas the abnormal variant was not detected in an American white population, suggesting the very rare occurrence of this variant in whites. Two common alleles, A and B, clearly identified in neuraminidase-treated samples, were observed at gene frequencies of 0.98 and 0.003, respectively, in the Japanese. These values are significantly different from the reported values in whites of 0.69 for A and 0.3 for B.
采用聚丙烯酰胺凝胶等电聚焦，随后进行酶谱分析和免疫固定，研究了日本人群和美国白人群体中各种纤溶酶原表型的频率分布。使用合成底物甲苯磺酰赖氨酸-α-萘酯进行酶谱分析，可在同一凝胶板上依次进行酶谱分析和免疫固定。通过这种方法，在血浆和血清中均很容易检测到一种无功能的异常纤溶酶原变体——富木纤溶酶原。该异常变体在日本人群中的基因频率为0.018，而在美国白人群体中未检测到这种异常变体，这表明该变体在白人中非常罕见。在经神经氨酸酶处理的样本中清晰鉴定出的两个常见等位基因A和B，在日本人中的基因频率分别为0.98和0.003。这些值与报道的白人中A为0.69、B为0.3的值有显著差异。
