Nakamura S, Abe K
Hum Genet. 1982;60(1):57-9. doi: 10.1007/BF00281264.
Genetic polymorphism of human plasminogen in the Japanese population has been described using polyacrylamide gel isoelectric focusing electrophoresis followed by immuno-fixation techniques. New variants PLG1'-1', PLG2'-1, and rare 1 were detected. Fibrinolytic activity per milligram plasminogen of each phenotype, except for PLG1'-1' and PLG1-1' was within the normal range. The PLG1' component was associated with no or less plasminogen activity, but possessed plasminogen antigen. Gene frequencies calculated from 750 individuals were PLG1;09560, PLG2; 0.0113, PLG1'; 0.0233, and PLG2',0.0094; respectively. The distribution of phenotypes fitted the Hardy-Weinberg equilibrium. In order to detect the plasminogen phenotypes the immunofixation technique was more suitable than the zymogram technique.
利用聚丙烯酰胺凝胶等电聚焦电泳结合免疫固定技术，对日本人群中的人纤溶酶原基因多态性进行了描述。检测到新的变异体PLG1'-1'、PLG2'-1和罕见型1。除PLG1'-1'和PLG1-1'外，各表型每毫克纤溶酶原的纤溶活性均在正常范围内。PLG1'组分的纤溶酶原活性无或较低，但具有纤溶酶原抗原。根据750名个体计算的基因频率分别为：PLG1为0.9560，PLG2为0.0113，PLG1'为0.0233，PLG2'为0.0094。表型分布符合哈迪-温伯格平衡。为了检测纤溶酶原表型，免疫固定技术比酶谱技术更合适。
