人血浆中的2',5'-寡腺苷酸和2',5'-寡腺苷酸磷酸二酯酶
2',5'-Oligoadenylate and 2',5'-oligoadenylate phosphodiesterase in human plasma.
作者信息
Sawai H, Ishibashi K, Itoh M, Watanabe S
出版信息
Biochem Biophys Res Commun. 1984 Dec 28;125(3):1061-6. doi: 10.1016/0006-291x(84)91391-3.
2',5'-Oligoadenylate and 2',5'-oligoadenylate phosphodiesterase activity were detected in the human plasma and serum by sensitive radioimmuno assays. The phosphodiesterase in the serum degraded 20 nM of added 2',5'-oligoadenylate in less than 1 hr. Addition of EDTA in the blood sample inhibited the phosphodiesterase activity completely and allowed the measurement of low levels of 2',5'-oligoadenylate. The concentration in the plasma from healty people was in the range of 0.03 to 0.3 nM.
通过灵敏的放射免疫分析法在人血浆和血清中检测到了2',5'-寡腺苷酸和2',5'-寡腺苷酸磷酸二酯酶活性。血清中的磷酸二酯酶在不到1小时内降解了添加的20 nM 2',5'-寡腺苷酸。在血样中添加乙二胺四乙酸（EDTA）可完全抑制磷酸二酯酶活性，并能测定低水平的2',5'-寡腺苷酸。健康人血浆中的浓度范围为0.03至0.3 nM。
Zotero 插件