Tatematsu M, Katsuyama T, Furihata C, Tsuda H, Ito N
Gan. 1984 Nov;75(11):957-65.
On the basis of paradoxical concanavalin A (Con A) staining, the tendency of tumor cells of gastric phenotype to shift to intestinal phenotype and the stability of the latter phenotype in stomach tumors of different sizes were examined quantitatively with an image processor. Phenotypic expression of tumors of the small intestine was also studied. One hundred male Wistar rats were given N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) at 50 micrograms/ml in their drinking water for 20 weeks (group 1). Twenty male F344 rats were given methylnitrosourea (MNU) at a dose of 50 mg/kg ip twice a week for 2 weeks (group 2). Rats in group 1 were killed in week 50 of the experiment and rats in group 2 were killed in week 25. In group 1, the percentage areas of intestinal-type cells in small, medium and large adenocarcinoma of the stomach were 0.5, 2.7 and 6.6%, the differences between these values being significant (P less than 0.05-0.01). Intestinal phenotypic expression of tumor cells of the stomach is stable and the proportion of intestinal-type cells in adenocarcinomas of the stomach is higher in the larger tumors. Adenomatous hyperplasias and adenocarcinomas of the small intestine in groups 1 and 2 were all composed entirely of cells of the intestinal type. These results suggested that intestinal-type cells in adenocarcinoma of the stomach did not originate from intestinal metaplasias but from gastric-type cells in stomach adenocarcinomas.
基于伴刀豆球蛋白A（Con A）的矛盾染色，使用图像处理器对胃表型肿瘤细胞向肠表型转变的趋势以及不同大小胃肿瘤中后一种表型的稳定性进行了定量研究。还研究了小肠肿瘤的表型表达。将100只雄性Wistar大鼠置于含有50微克/毫升N-甲基-N'-硝基-N-亚硝基胍（MNNG）的饮用水中20周（第1组）。将20只雄性F344大鼠以50毫克/千克的剂量腹腔注射甲基亚硝基脲（MNU），每周两次，共2周（第2组）。实验第50周处死第1组大鼠，第25周处死第2组大鼠。在第1组中，胃小、中、大腺癌中肠型细胞的面积百分比分别为0.5%、2.7%和6.6%，这些值之间的差异具有统计学意义（P<0.05-0.01）。胃肿瘤细胞的肠表型表达是稳定的，且在较大的胃腺癌中肠型细胞的比例更高。第1组和第2组中小肠的腺瘤样增生和腺癌均完全由肠型细胞组成。这些结果表明，胃腺癌中的肠型细胞并非源自肠化生，而是源自胃腺癌中的胃型细胞。
