The Giemsa-11 technique for species-specific chromosome differentiation. A simple stain modification leading to dependable direct and sequential staining procedures.

Oxidizing Methylene Blue and adding the reaction products to Eosin Y and Azure B makes possible a highly reliable Giemsa-11 technique for discrimination of chromosomes in hybrid cells according to their parental origin. This staining can be combined in a sequential procedure with a fluorescent banding technique allowing the exact identification of the chromosomes.