高效液相色谱法测定生物体液中阿司匹林及其代谢物的改进方法：在人类和动物研究中的应用
Improved method for the determination of aspirin and its metabolites in biological fluids by high-performance liquid chromatography: applications to human and animal studies.
作者信息
Mays D C, Sharp D E, Beach C A, Kershaw R A, Bianchine J R, Gerber N
出版信息
J Chromatogr. 1984 Nov 28;311(2):301-9. doi: 10.1016/s0378-4347(00)84723-4.
An improved method has been developed for the determination of acetylsalicylic acid, salicylic acid, gentisic acid, and salicyluric acid in plasma and urine of rabbits and man. Samples are extracted with dichloromethane containing mephenytoin as an internal standard, the solvent is evaporated under reduced pressure, the residue reconstituted and analyzed by high-performance liquid chromatography. Extraction efficiencies, linearity and assay precision were determined. This method has been applied to human bioavailability studies and the data are presented.
已开发出一种改进方法，用于测定兔和人的血浆及尿液中的乙酰水杨酸、水杨酸、龙胆酸和水杨尿酸。样品用含有美芬妥英作为内标的二氯甲烷萃取，溶剂在减压下蒸发，残留物复溶后用高效液相色谱分析。测定了萃取效率、线性和分析精密度。该方法已应用于人体生物利用度研究并给出了数据。
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