O'Kruk R J, Adams M A, Philp R B
J Chromatogr. 1984 Oct 12;310(2):343-52. doi: 10.1016/0378-4347(84)80099-7.
A rapid and sensitive high-performance liquid chromatographic technique was developed for the simultaneous determination of gentisic acid, salicyluric acid, acetylsalicylic acid and salicylic acid in plasma and serum. The method involved a single deproteinization step and separation using a reversed-phase column eluted with a buffered methanol (35%) mobile phase. Detection was achieved with a variable-wavelength ultraviolet detector set at 235 nm and a given chromatographic analysis could be completed in less than 10 min. The method was tested in both human and animal (rat) models given a single dose of acetylsalicylic acid.
开发了一种快速灵敏的高效液相色谱技术，用于同时测定血浆和血清中的龙胆酸、水杨尿酸、乙酰水杨酸和水杨酸。该方法包括单一的去蛋白步骤，并使用反相柱进行分离，用含35%甲醇的缓冲流动相洗脱。使用设定在235nm的可变波长紫外检测器进行检测，一次色谱分析可在不到10分钟内完成。该方法在给予单剂量乙酰水杨酸的人体和动物（大鼠）模型中进行了测试。
