Buskin J N, Upton R A, Williams R L
Clin Chem. 1982 May;28(5):1200-3.
Detailed performance specifications are given for a specific and sensitive liquid-chromatographic assay for aspirin, salicylic acid, and salicyluric acid in plasma. The sensitivity of this method for aspirin (50 micrograms/L) is 10-fold that of previous methods, so that concentrations of aspirin in plasma can now be followed for about four or five half-lives after the peak plasma concentration arising from a single 650-mg dose. In addition, this assay is as sensitive for salicylic and salicyluric acid in plasma as any hitherto. A modification permits measurement of gentisic acid, salicylic acid, and salicyluric acid in urine; further modifications allow indirect measurement of conjugated gentisate and salicylate in urine.
给出了一种用于测定血浆中阿司匹林、水杨酸和水杨尿酸的特定且灵敏的液相色谱分析方法的详细性能规格。该方法对阿司匹林的灵敏度（50微克/升）是先前方法的10倍，因此现在单次服用650毫克剂量后，血浆中阿司匹林的浓度在达到血浆峰值浓度后的四到五个半衰期内都可进行追踪。此外，该分析方法对血浆中的水杨酸和水杨尿酸的灵敏度与迄今任何方法一样高。一种改进方法可用于测定尿液中的龙胆酸、水杨酸和水杨尿酸；进一步的改进允许间接测定尿液中结合型龙胆酸盐和水杨酸盐。
