Ricci A, Arezzini C, Cocola F, Meiattini F
J Clin Microbiol. 1984 Dec;20(6):1065-7. doi: 10.1128/jcm.20.6.1065-1067.1984.
An automated method which uses oxidized Streptolysin O and determines the anti-Streptolysin O titer in whole blood without dilution of the sample is described. The addition of a reducing agent starts the hemolysis at an initial rate which is inversely proportional to the anti-Streptolysin O titer of the sample. An instrument (Taso-matic), designed to automatically execute all of the procedures, was used. Comparison between the described method and the classical hemolytic method gave the following linear regression slope y = 1.06x-13 (r = 0.974). Precision of the method at two different anti-Streptolysin O titers (200 and 500 IU), expressed as relative standard deviation, was 12.1 and 4.7% in the between-run procedure and 10.1 and 4.9% in the within-run procedure, respectively.
本文描述了一种自动化方法，该方法使用氧化型链球菌溶血素O，无需对样品进行稀释即可测定全血中的抗链球菌溶血素O滴度。加入还原剂后，溶血以初始速率开始，该速率与样品的抗链球菌溶血素O滴度成反比。使用了一种设计用于自动执行所有程序的仪器（Taso-matic）。所描述的方法与经典溶血法之间的比较得出以下线性回归斜率y = 1.06x - 13（r = 0.974）。该方法在两种不同抗链球菌溶血素O滴度（200和500 IU）下的精密度，以相对标准偏差表示，批间程序分别为12.1%和4.7%，批内程序分别为10.1%和4.9%。
