Lynes M A, Flaherty L, Michaelson J, Collins J J, Rinchik E M
J Immunogenet. 1984 Jun-Aug;11(3-4):189-96. doi: 10.1111/j.1744-313x.1984.tb01056.x.
Normal C57BL/6 (B6) mouse serum was tested in the direct cytotoxicity assay for specific reactivity against lipopolysaccharide (LPS)-stimulated mouse spleen cells. Selective reactivity was found in weanling and adult serum against lymphoblasts from mice that express an antigen encoded by the H-2Kk region of the major histocompatibility complex (MHC). Other strains, congenic with B6 at the MHC, did not exhibit the same alloreactivity. Serum from mice of a congenic strain being derived in our laboratory, which differs from B6 at two unlinked loci, Tla and nu, exhibited similar reactivity against the B6-H-2k LPS-stimulated lymphoblasts, implying that a competent T cell compartment is not necessary for generation of this reactivity. Such reactivities may result from environmental stimulation of the immune system, from internal immunoregulatory controls, or from some combination of these immune stimuli.
在直接细胞毒性试验中，检测了正常C57BL/6（B6）小鼠血清对脂多糖（LPS）刺激的小鼠脾细胞的特异性反应性。在断奶小鼠和成年小鼠血清中发现了对表达主要组织相容性复合体（MHC）H-2Kk区域编码抗原的小鼠淋巴母细胞的选择性反应性。在MHC上与B6同基因的其他品系未表现出相同的同种异体反应性。我们实验室衍生的一个同基因品系小鼠的血清，在两个不连锁的位点Tla和nu上与B6不同，对B6-H-2k LPS刺激的淋巴母细胞表现出相似的反应性，这意味着产生这种反应性不需要有功能的T细胞区室。这种反应性可能是由免疫系统的环境刺激、内部免疫调节控制或这些免疫刺激的某种组合引起的。
