Ivanova L A, Abramova T A, Levkovich N M, Okhotnikova V F, Kartashov V S
Antibiotiki. 1984 Dec;29(12):922-4.
The results of the comparative study on microbiological and chemical quantitative determination of kanamycin sulfate in the ophthalmic films with the collagen base are presented. The intraocular films prepared with the use of 1 per cent collagen solution contain dexamethasone and kanamycin. The agar diffusion method with Bacillus pumilus NCTC 8241 as the test microbe and the photocolorimetric method based on estimation of the optical density of the colored compound formed after acid hydrolysis of kanamycin with orcinol and ferric chloride were used for the quantitative determination of kanamycin. The results of the quantitative determinations of kanamycin in the films with the two methods did not differ significantly. However, the error of the microbiological method was +/- 3,75 per cent, whereas that of the chemical method was +/- 1.23 per cent or approximately 3 times lower. The time of the analysis decreased from 24 to 1.5-2 h. Moreover, the chemical method is simple and readily reproducible.
本文介绍了对含胶原蛋白基质的眼科薄膜中硫酸卡那霉素进行微生物学和化学定量测定的比较研究结果。使用1%胶原蛋白溶液制备的眼内薄膜含有地塞米松和卡那霉素。以短小芽孢杆菌NCTC 8241作为测试微生物的琼脂扩散法，以及基于对卡那霉素与苔黑酚和氯化铁酸水解后形成的有色化合物的光密度估计的分光比色法，用于卡那霉素的定量测定。两种方法对薄膜中卡那霉素的定量测定结果无显著差异。然而，微生物学法的误差为±3.75%，而化学法的误差为±1.23%，约低3倍。分析时间从24小时缩短至1.5 - 2小时。此外，化学法简单且易于重现。
