Terada T, Nakanuma Y
Second Department of Pathology, Kanazawa University School of Medicine, Japan.
Hepatology. 1994 Aug;20(2):388-97.
We investigated expression of carbohydrate chain structures during human intrahepatic bile duct development and maturation. Results of staining for ABH antigens were negative in the ductal plate and biliary cells migrating into the mesenchyma but were positive in newly formed bile ducts in the fetal liver and in maturing bile ducts in the postnatal liver. Staining for Lewis(a), sialyl Lewis(a) and Lewisb antigens was negative in the ductal plate but positive in migrating biliary cells, newly formed ducts and maturing ducts. Staining for sialyl Lewisx antigen was positive in the ductal plate, migrating biliary cells and newly formed ducts but negative in the postnatal liver. Lewis(y) antigen staining was negative in the ductal plate and migrating biliary cells but positive in newly formed ducts and maturing ducts. Staining for T and Tn antigens was negative in the ductal plate, migrating biliary cells and newly formed ducts but positive in maturing ducts. Staining for Lewisx, sialyl Tn and carcinoembryonic antigen was negative in all tissues. Receptors of Ulex europaeus agglutinin I and soybean agglutinin were absent in the ductal plate, migrating biliary cells and newly formed ducts but present in maturing large ducts. Staining for receptors of concanavalin A and Ricnus communis agglutinin I was negative in the ductal plate but positive in migrating biliary cells, newly formed ducts and maturing ducts. Staining for the receptor of succinylated wheat germ agglutinin was always positive. These findings suggest that carbohydrate chain structures change during intrahepatic bile duct development and maturation and that new carbohydrate chain structures gradually emerge as intrahepatic bile ducts develop and mature.
我们研究了人肝内胆管发育和成熟过程中碳水化合物链结构的表达。ABH抗原染色结果显示，在导管板和迁移至间充质的胆管细胞中呈阴性，但在胎儿肝脏中新形成的胆管以及出生后肝脏中成熟的胆管中呈阳性。Lewis(a)、唾液酸化Lewis(a)和Lewisb抗原染色在导管板中呈阴性，但在迁移的胆管细胞、新形成的胆管和成熟的胆管中呈阳性。唾液酸化Lewisx抗原染色在导管板、迁移的胆管细胞和新形成的胆管中呈阳性，但在出生后肝脏中呈阴性。Lewis(y)抗原染色在导管板和迁移的胆管细胞中呈阴性，但在新形成的胆管和成熟的胆管中呈阳性。T和Tn抗原染色在导管板、迁移的胆管细胞和新形成的胆管中呈阴性，但在成熟的胆管中呈阳性。Lewisx、唾液酸化Tn和癌胚抗原染色在所有组织中均呈阴性。欧洲荆豆凝集素I和大豆凝集素的受体在导管板、迁移的胆管细胞和新形成的胆管中不存在，但在成熟的大胆管中存在。伴刀豆球蛋白A和蓖麻凝集素I受体的染色在导管板中呈阴性，但在迁移的胆管细胞、新形成的胆管和成熟的胆管中呈阳性。琥珀酰化小麦胚凝集素受体的染色始终呈阳性。这些发现表明，碳水化合物链结构在肝内胆管发育和成熟过程中发生变化，并且随着肝内胆管的发育和成熟，新的碳水化合物链结构逐渐出现。
