Madrid J F, Ballesta J, Galera T, Castells M T, Pérez-Tomás R
Department of Cell Biology, School of Medicine, University of Murcia, Spain.
Histochemistry. 1989;91(5):437-43. doi: 10.1007/BF00493832.
A battery of seven lectins and several conventional mucin histochemical techniques were used to identify the epithelial mucins of the gallbladder of ten species: man, rabbit (Oryctolagus cuniculus, mammalia), hamster (Mesocricetus auratus, mammalia), chicken (Gallus gallus, bird), sparrow (Passer domesticus, bird), moorish gecko (Tarentola mauritanica, reptilia), ladder snake (Elaphe scalaris, reptilia), lake frog (Rana perezi, amphibia), natterjack toad (Bufo calamita, amphibia) and gilthead sea bream (Sparus auratus, fish). Glycogen was found in the epithelial lining of the reptilian and amphibian gallbladders. Sulphate and carboxyl groups were frequently found in the same species, except in the ladder snake and natterjack toad gallbladders where only sulphate groups were identified. Sialic acid residues were detected in man, rabbit, bird, T. mauritanica, R. perezi and fish gallbladders. ConA binding pattern was similar in the ten species studied. In the human gallbladder only PNA failed to label the luminal surface, while the glands were only unreactive to DBA. The human gallbladder showed a large variety of saccharides. The present results suggest that no relation exists between the composition of the gallbladder mucins and the situation of the species in the phylogenetic scale.
采用一组七种凝集素和几种传统的黏蛋白组织化学技术，对十种物种的胆囊上皮黏蛋白进行鉴定：人类、兔子（穴兔，哺乳纲）、仓鼠（金黄仓鼠，哺乳纲）、鸡（原鸡，鸟纲）、麻雀（家麻雀，鸟纲）、摩尔壁虎（摩洛哥壁虎，爬行纲）、锦蛇（花脊游蛇，爬行纲）、湖蛙（佩氏林蛙，两栖纲）、黄条蟾蜍（绿蟾蜍，两栖纲）和金头鲷（金头鲷，鱼纲）。在爬行类和两栖类动物的胆囊上皮衬里中发现了糖原。除了锦蛇和黄条蟾蜍的胆囊中仅鉴定出硫酸基团外，在同一物种中经常同时发现硫酸基团和羧基。在人类、兔子、鸟类、摩洛哥壁虎、佩氏林蛙和鱼类的胆囊中检测到唾液酸残基。在所研究的十个物种中，伴刀豆球蛋白A的结合模式相似。在人类胆囊中，只有花生凝集素未能标记管腔表面，而腺体仅对双花扁豆凝集素无反应。人类胆囊显示出多种糖类。目前的结果表明，胆囊黏蛋白的组成与物种在系统发育尺度上的位置之间不存在关联。
