Causon R C, Brown M J
J Chromatogr. 1984 Dec 28;317:319-25. doi: 10.1016/s0021-9673(01)91670-x.
A method for the measurement of tyramine in human plasma is described. It is based on tetraphenylboron ion-pair extraction and reversed-phase ion-pairing liquid chromatography with amperometric detection. Tyramine can be reliably measured in the range 5-200 ng/ml with an absolute limit of detection of 0.50 ng/ml at a signal-to-noise ratio of 2.0. Correction for variable recovery is made by using a tritiated tyramine internal standard. This assay is suitable for studies on the bioavailability of ingested tyramine and should thus have a role in the development of safer monoamine oxidase inhibitor drugs.
本文描述了一种测量人体血浆中酪胺的方法。该方法基于四苯基硼离子对萃取和反相离子对液相色谱，并采用安培检测。酪胺在5-200 ng/ml范围内能够可靠地测量，在信噪比为2.0时，绝对检测限为0.50 ng/ml。通过使用氚标记的酪胺内标来校正回收率的变化。该测定法适用于研究摄入酪胺的生物利用度，因此在开发更安全的单胺氧化酶抑制剂药物方面应能发挥作用。
