采用液相色谱-电化学检测法测定尿中3-甲氧基-4-羟基苯乙二醇。
Determination of urinary 3-methoxy-4-hydroxyphenylglycol by liquid chromatography with electrochemical detection.
作者信息
Koch D D, Polzin G
出版信息
J Chromatogr. 1984 Dec 28;317:327-31. doi: 10.1016/s0021-9673(01)91671-1.
The increasing popularity of urinary 3-methoxy-4-hydroxyphenylglycol (MHPG) determinations has placed a demand on clinical laboratories to provide these data. Previous methods for MHPG either exhibit unacceptable performance, are too slow, or utilize instrumentation not commonly employed in these laboratories. The liquid chromatography electrochemical detection method described here has several positive features. MHPG is isolated by extraction, separated by liquid chromatography, and quantitated at an amperometric electrode. The precision, simplicity, and relatively low cost makes this new method an ideal choice for the clinical laboratory.
尿中3-甲氧基-4-羟基苯乙二醇（MHPG）测定日益普及，这就要求临床实验室提供这些数据。以往的MHPG检测方法要么性能不佳，要么速度过慢，要么使用这些实验室不常用的仪器。本文所述的液相色谱电化学检测法具有几个积极的特点。MHPG通过萃取分离，经液相色谱分离后，在安培电极上进行定量分析。该方法的精密度、简便性以及相对较低的成本使其成为临床实验室的理想选择。
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