Raines D E, Cafiso D S
J Membr Biol. 1984;82(3):241-7. doi: 10.1007/BF01871633.
Fluorescent, dansyl derivatives of triphenylalkylphosphonium ions have been synthesized and exhibit fluorescence intensities in small sonicated phospholipid vesicles that are dependent upon transmembrane potentials. The voltage-dependent fluorescence changes are a result of changes in quantum yield that accompany a voltage-dependent phase partitioning of the probe. This phase partitioning is easily quantitated by calibrating the intensities of totally membrane-associated and aqueous probe. The voltage-dependence is well accounted for by a simple thermodynamic model and allows an estimation of potentials from fluorescence intensities in small vesicle systems.
已合成了三苯基烷基鏻离子的荧光丹磺酰衍生物，其在小型超声处理的磷脂囊泡中呈现出依赖于跨膜电位的荧光强度。电压依赖性荧光变化是量子产率变化的结果，这种变化伴随着探针的电压依赖性相分配。通过校准完全与膜结合的探针和水相探针的强度，可以很容易地对这种相分配进行定量。简单的热力学模型很好地解释了电压依赖性，并允许从小型囊泡系统中的荧光强度估计电位。
