Harabuchi I, Kishi R, Ikeda T, Kiyosawa H, Miyake H
Sangyo Igaku. 1984 Jul;26(4):279-82. doi: 10.1539/joh1959.26.279.
An experimental study of toluene exposure was performed on mice at various times of the day using the righting reflex and blood concentration as indicators. One hundred and sixty ICR mice were housed 8 mice per cage for 3 weeks with free access to food and water under a dark-light cycle consisting of alternate 12 hr periods of darkness and light: the dark period beginning at 7:00. The exposure was begun when the mice were 8 weeks old. They were exposed to 10,000 ppm of toluene for 40 min at 4:00, 10:00, 16:00 and 22:00, and they were sacrificed at the end of the exposure, after 3 min or after 30 min. The duration of anesthesia was 200.2 +/- 15.4 (sec) at 4:00, 361.1 +/- 21.5 (sec) at 10:00, 335.1 +/- 18.5 (sec) at 16:00 and 309.2 +/- 19.8 (sec) at 22:00. The duration of anesthesia at 4:00 was shorter than that of the other times of the day statistically (p less than 0.01). With regard to the toluene concentration in blood, there was no significant difference between those mice sacrificed at the end of exposure and those sacrificed after 3 min.
以翻正反射和血液浓度为指标，在一天中的不同时间对小鼠进行甲苯暴露的实验研究。160只ICR小鼠，每笼饲养8只，共饲养3周，在由12小时黑暗和12小时光照交替组成的明暗循环条件下自由进食和饮水，黑暗期从7:00开始。小鼠8周龄时开始暴露实验。分别于4:00、10:00、16:00和22:00让小鼠暴露于10000 ppm的甲苯中40分钟，暴露结束时、暴露后3分钟或30分钟后将小鼠处死。4:00时麻醉持续时间为200.2±15.4（秒），10:00时为361.1±21.5（秒），16:00时为335.1±18.5（秒），22:00时为309.2±19.8（秒）。4:00时的麻醉持续时间在统计学上短于一天中的其他时间（p＜0.01）。关于血液中的甲苯浓度，暴露结束时处死的小鼠与暴露后3分钟处死的小鼠之间没有显著差异。
