Seggie J, Werstiuk E S, Grota L, Joshi M, Brown G M
Prog Neuropsychopharmacol Biol Psychiatry. 1984;8(4-6):733-6. doi: 10.1016/0278-5846(84)90048-4.
Lithium has been suggested to exert some of its theraputic effects by modifying the function of the retinal-hypothalamic pineal pathway that is essential for the chronobiology of an organism (Seggie et al., 1983). Previous work was done in Wistar rats, an Albino species which lacks the enzyme for synthesis of eye pigment. This pigment is important in the regulation of light cued rhythms. The present project investigated effects of lithium in a pigmented eye strain. Adult male Long Evans rats were maintained individually on a 12 hour light/12 hour dark schedule with free access to water and one of three diets: (1) normal laboratory chow; (2) a low lithium diet: lab chow supplemented with 50 mM/kg of lithium chloride. Body weight and water intake were measured after six weeks on the diets. In Experiment I, separate groups of rats were sacrificed by rapid decapitation every 4 hours in the light/dark cycle. In Experiment II, animals were sacrificed every 90 min. between 12:00 and 20:00 hours during the dark cycle. Blood and pineal glands were collected for lithium determination and assay of melatonin by RIA. In Experiment I, plasma lithium levels were 0.35 +/- 0.01 and 0.57 +/- 0.02 mEq/1 for the low and high diets. Serum and pineal melatonin evidenced the expected diurnal rhythms. The diets had no effect on these parameters except at 14:00 hours.(ABSTRACT TRUNCATED AT 250 WORDS)
锂被认为通过改变视网膜 - 下丘脑 - 松果体通路的功能来发挥其部分治疗作用，该通路对生物体的生物钟学至关重要（Seggie等人，1983年）。先前的研究是在Wistar大鼠身上进行的，这是一种缺乏眼色素合成酶的白化物种。这种色素在光导节律的调节中很重要。本项目研究了锂在有色眼品系中的作用。成年雄性Long Evans大鼠以12小时光照/12小时黑暗的时间表单独饲养，可自由饮水，并可选择三种饮食之一：（1）正常实验室饲料；（2）低锂饮食：添加50 mM/kg氯化锂的实验室饲料。在喂食六周后测量体重和饮水量。在实验I中，在光/暗周期中每4小时通过快速断头处死不同组的大鼠。在实验II中，在黑暗周期的12:00至20:00之间每90分钟处死动物。收集血液和松果体用于锂测定以及通过放射免疫分析测定褪黑素。在实验I中，低饮食和高饮食组的血浆锂水平分别为0.35±0.01和0.57±0.02 mEq/1。血清和松果体褪黑素呈现出预期的昼夜节律。除了在14:00时，饮食对这些参数没有影响。（摘要截断于250字）
