Werstiuk E S, Seggie J, Joshi M
Prog Neuropsychopharmacol Biol Psychiatry. 1984;8(4-6):765-8. doi: 10.1016/0278-5846(84)90055-1.
Lithium has been implicated in chronobiology (Seggie, et al., 1982, 1983). Previous work was done in Wistar rats, an Albino species, which lack the enzyme for synthesis of eye pigment. This pigment is important in regulation of light cued rhythms. The present project extends observations to Long Evans rats, a strain which has a pigmented eye. Groups of adult male rats were fed one of three diets: (1) normal laboratory chow; (2) a low lithium diet: lab chow supplemented with 30 mM/kg lithium chloride; and (3) a high lithium diet: lab chow supplemented with 50 mM/kg lithium chloride. Adult male rats were house individually with free access to diet and water under a 12 hour light/12 hour dark schedule. After six weeks on the diets, body weight, water intake, plasma, red blood cell and retinal lithium and retinal weight were measured every 4 hours throughout the 24 hour cycle following sacrifice by rapid decapitation. Plasma lithium levels on the high diet were 0.57 +/- 0.02 mEq/l and did not evidence a diurnal rhythm. Plasma lithium levels on the low diet were 0.35 +/- 0.01 mEq/l and evidenced a small, but significant diurnal rhythm with levels lowest just before darkness and normal food intake. Red blood cell lithium levels were significantly higher than plasma levels at 0.83 +/- 0.02 and 0.52 +/- 0.02 mEq/l for the high and low diets. Low diet, but not high diet red blood cells lithium levels evidenced a significant rhythm with a pattern similar to that seen in plasma lithium levels.(ABSTRACT TRUNCATED AT 250 WORDS)
锂与生物钟学有关（塞吉等人，1982年，1983年）。之前的研究是在Wistar大鼠身上进行的，这是一种白化物种，缺乏合成眼色素的酶。这种色素在调节光信号节律方面很重要。本项目将观察范围扩展到长 Evans 大鼠，这是一种眼睛有色素的品系。成年雄性大鼠被分成几组，分别喂食三种饮食之一：（1）正常实验室饲料；（2）低锂饮食：添加了30 mM/kg 氯化锂的实验室饲料；（3）高锂饮食：添加了50 mM/kg 氯化锂的实验室饲料。成年雄性大鼠单独饲养，在12小时光照/12小时黑暗的时间表下可自由获取食物和水。在喂食六周后，通过快速断头处死大鼠，然后在整个24小时周期内每4小时测量一次体重、饮水量、血浆、红细胞和视网膜中的锂含量以及视网膜重量。高锂饮食组的血浆锂水平为0.57±0.02 mEq/l，未显示出昼夜节律。低锂饮食组的血浆锂水平为0.35±0.01 mEq/l，显示出一个小但显著的昼夜节律，在接近黑暗和正常进食前水平最低。高锂和低锂饮食组的红细胞锂水平分别显著高于血浆水平，为0.83±0.02和0.52±0.02 mEq/l。低锂饮食组的红细胞锂水平显示出显著的节律，模式与血浆锂水平相似，而高锂饮食组则没有。（摘要截取自250字）
