Lewerenz H J, Plass R
Arch Toxicol. 1984 Dec;56(2):92-5. doi: 10.1007/BF00349078.
The effects of ethylenethiourea (ETU) on the hepatic xenobiotic metabolizing system in rats and mice were investigated. Male rats and male mice were given oral doses of 50 and 75, or 50, 75, 100, 500, and 1,000 mg/kg for 3 days. The microsomal enzymes studied were aminopyrine N-demethylase, aniline hydroxylase, and cytochrome P-450. In rats, the activity of aminopyrine N-demethylase was reduced to values between 60 and 70% of controls 24 h after treatment. A decrease in aniline hydroxylase activity and cytochrome P-450 content was observed on the 3rd day after exposure. In mice, treatment with ETU resulted in an increase of cytochrome P-450 at all dose levels. The activity of aniline hydroxylase was significantly elevated in the groups receiving doses of 100 mg/kg and higher. Aminopyrine N-demethylase was unaffected by the treatment. The results suggest that there are qualitative differences between rats and mice after ETU exposure with respect to the response of the hepatic mono-oxygenases.
研究了乙撑硫脲（ETU）对大鼠和小鼠肝脏外源性物质代谢系统的影响。给雄性大鼠和雄性小鼠口服剂量为50和75，或50、75、100、500和1000mg/kg，持续3天。所研究的微粒体酶有氨基比林N-脱甲基酶、苯胺羟化酶和细胞色素P-450。在大鼠中，治疗后24小时，氨基比林N-脱甲基酶的活性降至对照组的60%至70%。暴露后第3天观察到苯胺羟化酶活性和细胞色素P-450含量降低。在小鼠中，用ETU治疗导致所有剂量水平的细胞色素P-450增加。接受100mg/kg及更高剂量的组中苯胺羟化酶的活性显著升高。氨基比林N-脱甲基酶不受治疗影响。结果表明，ETU暴露后，大鼠和小鼠在肝脏单加氧酶反应方面存在质的差异。
