Tavassoli M
Scand J Haematol. 1978 Apr;20(4):330-4. doi: 10.1111/j.1600-0609.1978.tb02464.x.
The behavior of isolated, disaggregated white adipose cells obtained from marrow and extramedullar sites in the rabbit was compared in monolayer cultures. Both cell types transform within 2 weeks into fibroblast-like cells which can be maintained in subcultures. Both in freshly isolated state and in fibroblast-like state the marrow adipocyte contains esterase activities reacting with naphthol AS acetate and D-chloracetate. These activites are not present in extramedullary cells. In addition, alpha-naphthyl butyrate-reacting esterase, present in both cell types, is fluoride-resistant in marrow adipocytes but labile in extramedullar cells. These differences are consistent with heterogeneity of white adipose tissue and suggest different potentials for marrow and extramedullary adipocytes.
在单层培养中比较了从兔骨髓和髓外部位获得的分离、分散的白色脂肪细胞的行为。两种类型的细胞在2周内都会转变为成纤维细胞样细胞，并可进行传代培养。无论是在新鲜分离状态还是成纤维细胞样状态下，骨髓脂肪细胞都含有与醋酸萘酚AS和D-氯醋酸反应的酯酶活性。这些活性在髓外细胞中不存在。此外，两种细胞类型中都存在的α-萘丁酸反应性酯酶，在骨髓脂肪细胞中对氟化物有抗性，但在髓外细胞中不稳定。这些差异与白色脂肪组织的异质性一致，并表明骨髓和髓外脂肪细胞具有不同的潜能。
