一种用于实验性动脉血栓形成研究的标准化“体内”模型：方法描述

A standardized 'in vivo' model for the study of experimental arterial thrombosis: description of a method.

作者信息

Bourgain R H, Vermariën H, Andries R, Vereecke F, Jacqueloot J, Rennies J, Blockeel E, Six F

出版信息

Adv Exp Med Biol. 1984;180:635-49. doi: 10.1007/978-1-4684-4895-5_62.

Abstract

A method for "in vivo" induction and registration of arterial platelet thrombosis has been developed and standardized in a branch of the mesenteric artery of the white Wistar rat. It consists in local deendothelialization by electrical current. Thrombus induction is performed by topical superfusion with ADP; when the superfusion is discontinued the thrombus disappears but can again be induced reproducibly by renewal of the ADP superfusion after a time interval not exceeding 15 minutes. Registration of the thrombotic phenomenon is made possible by microprojection of the investigated arterial segment. An appropriate optoelectronic device allows the on-line derivation of discriminating parameters. Off-line storage and processing of the experimental data by computer is provided. The method is automatized and can easily be applied to other species of laboratory animals.

摘要

一种用于“体内”诱导和记录动脉血小板血栓形成的方法已在白色Wistar大鼠肠系膜动脉的一个分支中开发并标准化。它包括通过电流进行局部内皮剥脱。血栓诱导通过ADP局部灌注进行；当停止灌注时，血栓消失，但在不超过15分钟的时间间隔后，通过重新进行ADP灌注可再次可重复诱导血栓形成。通过对所研究动脉段进行显微投影，可以记录血栓形成现象。一种合适的光电装置可在线导出鉴别参数。提供了通过计算机对实验数据进行离线存储和处理的功能。该方法是自动化的，并且可以很容易地应用于其他实验室动物物种。