Butcher B T, Salvaggio J E, O'Neil C E, Weill H, Garg O
J Allergy Clin Immunol. 1977 Mar;59(3):223-7. doi: 10.1016/0091-6749(77)90153-1.
Selected workers exhibiting clinical "sensitivity" to toluene diiosocyanate (TDI) (wheezing, cough, and dyspnea upon entering a TDI-containing area) were studied for : (1) in vitro TDI-induced leukocyte histamine release; (2) determination of cyclic 3',5' adenosine monophosphate (cAMP) levels of lymphocytes exposed to TDI; (3) effect of TDI on the isoproterenol-induced increase of lymphocyte cAMP levels: and (4) acetyl-beta-methylcholine (mecholyl) inhalation challenge. TDI did not induce histamine release from leukocytes of "sensitive" or "nonsensitive" individuals, nor were lymphocyte cAMP levels affected by in vitro TDI exposure, TDI did, however, diminish in vitro stimulation of cAMP by isoproterenol. This effect, seen with cells of "sensitive" and "nonsensitive" individuals, appeared to be dose-dependent; there were no significant differences between the two groups. When challenged with mecholyl, 7 of 10 "sensitive" but only 1 of 10 "nonsensitive" individuals showed a greater than 20% decrease in FEV1. These results suggest that TDI-induced obstructive airways disorders may be associated with altered beta-adrenergic function.
对选定的表现出对甲苯二异氰酸酯（TDI）有临床“敏感性”（进入含TDI区域后出现喘息、咳嗽和呼吸困难）的工人进行了以下研究：（1）体外TDI诱导的白细胞组胺释放；（2）测定暴露于TDI的淋巴细胞中环3',5'-腺苷单磷酸（cAMP）水平；（3）TDI对异丙肾上腺素诱导的淋巴细胞cAMP水平升高的影响；以及（4）乙酰-β-甲基胆碱（乙酰甲胆碱）吸入激发试验。TDI不会诱导“敏感”或“不敏感”个体的白细胞释放组胺，体外暴露于TDI也不会影响淋巴细胞的cAMP水平，然而，TDI确实会减弱异丙肾上腺素对cAMP的体外刺激作用。这种作用在“敏感”和“不敏感”个体的细胞中均可见，且似乎呈剂量依赖性；两组之间无显著差异。当用乙酰甲胆碱进行激发试验时，10名“敏感”个体中有7名FEV1下降超过20%，而10名“不敏感”个体中只有1名出现这种情况。这些结果表明，TDI诱导的阻塞性气道疾病可能与β-肾上腺素能功能改变有关。
