Effect of variation of in vitro culture techniques upon oocyte fertilization and embryo development in human in vitro fertilization procedures.

Human in vitro fertilization requires a safe healthy environment for gamete interaction. This report compares the use of tube cultures with that of organ culture dishes for human in vitro fertilization procedures. Moreover, an oocyte grading system is described that provides an individualized approach for timing of insemination. From the results of this study, the use of organ culture dishes and variable oocyte preincubation demonstrates a significant improvement in human fertilization success in vitro.