Edgar D H, Whalley K M, Mills J A
J In Vitro Fert Embryo Transf. 1987 Apr;4(2):111-5. doi: 10.1007/BF01555450.
The early embryonic development of in vitro fertilized oocytes was assessed following superovulation in F1 hybrid (C57BL/6 X CBA/Ca) mice. Decreasing the time interval between the administration of constant doses of pregnant mare's serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) resulted in decreases in the frequency of development to the blastocyst stage but had no significant effect on development to the two-cell stage. Preincubation of postovulatory oocytes in vitro prior to insemination did not compensate for the reduced preovulatory development in vivo but resulted in decreases in the frequency of development to the blastocyst stage. The results indicate that inadequate preovulatory development of superovulated mouse oocytes can adversely affect the preimplantation development of in vitro fertilized embryos in the absence of a visible inhibitory effect on development to the two-cell stage and also that preincubation of postovulatory oocytes in vitro prior to fertilization reduces subsequent developmental capacity.
在F1杂交（C57BL/6×CBA/Ca）小鼠超排卵后，评估体外受精卵母细胞的早期胚胎发育情况。缩短恒量孕马血清促性腺激素（PMSG）和人绒毛膜促性腺激素（hCG）给药之间的时间间隔，会导致发育至囊胚阶段的频率降低，但对发育至二细胞阶段没有显著影响。排卵后卵母细胞在体外受精前进行预孵育，不能弥补体内排卵前发育的不足，反而会导致发育至囊胚阶段的频率降低。结果表明，超排卵小鼠卵母细胞排卵前发育不足，在对二细胞阶段发育没有明显抑制作用的情况下，会对体外受精胚胎的着床前发育产生不利影响，而且受精前排卵后卵母细胞在体外预孵育会降低后续的发育能力。
