在Percoll梯度上分离不含血细胞的活微丝蚴。
Separation of viable microfilariae free of blood cells on Percoll gradients.
作者信息
Chandrashekar R, Rao U R, Rajasekariah G R, Subrahmanyam D
出版信息
J Helminthol. 1984 Mar;58(1):69-70. doi: 10.1017/s0022149x00028078.
PMID:6538892
Abstract
A consistent and reproducible method is described for isolating pure populations of microfilariae of Litomosoides carinii, Brugia pahangi, B. malayi and Dipetalonema viteae, free of cells, from blood, by density gradient centrifugation on Percoll in 0.25 M sucrose. The recovery of the microfilariae was 85 to 97%.
摘要
本文描述了一种一致且可重复的方法，通过在0.25M蔗糖中的Percoll上进行密度梯度离心，从血液中分离出纯净的卡氏丝状线虫、彭亨布鲁线虫、马来布鲁线虫和旋盘尾丝虫微丝蚴群体，且不含细胞。微丝蚴的回收率为85%至97%。
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