Simmonds A P, McDonald E C
Br J Cancer. 1984 Sep;50(3):317-26. doi: 10.1038/bjc.1984.178.
Ovarian tumours were cultured by clonogenic assay and drug sensitivity profiles obtained for cis-platinum, adriamycin and phosphoramide mustard. Results were correlated with clinical outcome. Two hundred samples were received from 106 patients and 115/167 with malignant cytology (69%) were cultured successfully. Drug results were obtained on 71 samples and in untreated patients 60% of samples (48% of patients) were markedly sensitive to cis-platinum and 87% of samples (76% of patients) were sensitive to adriamycin. Eighty-one percent of cases sensitive to adriamycin were also sensitive to cis-platinum. Two of 7 samples were sensitive to phosphoramide mustard; the remainder were resistant. Eighty percent of samples from treated patients were resistant in vitro to drugs already received. Seventy-one samples from 57 patients were suitable for drug study. Forty-eight patients received chemotherapy, but only 23 received the drugs tested. Clinical correlations showed that in vitro sensitivity to cis-platinum and adriamycin was related to a good clinical response. No correlations were observed between cis-platinum and adriamycin resistance in vitro and clinical outcome. Unexpected relationships, however, were observed between cis-platinum resistance and failure to respond to other alkylating agents received singly. No such relationship has been demonstrated for adriamycin.
通过克隆形成试验培养卵巢肿瘤，并获得顺铂、阿霉素和环磷酰胺的药敏谱。结果与临床结果相关。从106例患者中获取了200份样本，其中115/167例恶性细胞学样本（69%）培养成功。对71份样本进行了药敏检测，在未经治疗的患者中，60%的样本（48%的患者）对顺铂明显敏感，87%的样本（76%的患者）对阿霉素敏感。对阿霉素敏感的病例中81%对顺铂也敏感。7份样本中有2份对环磷酰胺敏感；其余均耐药。来自接受过治疗患者的80%的样本在体外对已接受的药物耐药。来自57例患者的71份样本适合进行药敏研究。48例患者接受了化疗，但只有23例接受了所检测的药物。临床相关性表明，体外对顺铂和阿霉素的敏感性与良好的临床反应相关。未观察到体外顺铂和阿霉素耐药与临床结果之间的相关性。然而，观察到顺铂耐药与对单独使用的其他烷化剂无反应之间存在意外关系。阿霉素未显示出这种关系。
