Lord P F, Kapp D S, Hayes T, Weshler Z
Eur J Cancer Clin Oncol. 1984 Aug;20(8):1079-85. doi: 10.1016/0277-5379(84)90111-1.
The design of clinical trials employing whole-body hyperthermia in cancer therapy has been hampered due to lack of a suitable animal model. We describe a technique for reproducibly and efficiently inducing whole-body hyperthermia in Sprague-Dawley rats, using halothane and oxygen anesthesia and immersion in a hot water bath. Core body temperatures of between 41.5 and 43 degrees C were induced and maintained for periods of up to 200 min and survival curves were determined. The time of exposure at a given temperature that resulted in death in 50% of the animals within 24 hr after heating (LD50/24 hr) was calculated by linear logistic regression analysis. LD50 24 hr values of 115, 61, 57, 25 and 16 min were obtained for temperatures of 41.75, 42.0, 42.25, 42.5 and 42.75 degrees C respectively. This heating technique is compared to several more toxic methods for inducing whole-body hyperthermia with respect to possible pharmacological and physiological differences.
由于缺乏合适的动物模型，采用全身热疗进行癌症治疗的临床试验设计受到了阻碍。我们描述了一种在Sprague-Dawley大鼠中可重复且高效地诱导全身热疗的技术，该技术使用氟烷和氧气麻醉，并将大鼠浸入热水浴中。诱导并维持核心体温在41.5至43摄氏度之间长达200分钟，并测定生存曲线。通过线性逻辑回归分析计算在给定温度下暴露的时间，该时间会导致50%的动物在加热后24小时内死亡（LD50/24小时）。对于41.75、42.0、42.25、42.5和42.75摄氏度的温度，分别获得的LD50 24小时值为115、61、57、25和16分钟。就可能的药理学和生理学差异而言，将这种加热技术与几种毒性更强的诱导全身热疗的方法进行了比较。
