Rofstad E K, Solesvik O V, Brustad T
Eur J Cancer Clin Oncol. 1984 Oct;20(10):1295-1305. doi: 10.1016/0277-5379(84)90259-1.
The effect of hyperthermia at 42.5 degrees C on a human melanoma xenograft in athymic mice was studied. The tumours were heated in vivo in a water-bath. Tumour growth delay and single-cell survival in vitro were used as endpoints. Qualitative information regarding heat-induced vascular damage was obtained from microangiographic analysis. Tumour growth delay after a given treatment was considerably longer than that expected from the cell survival measured in vitro immediately after treatment. Experiments in which removal of the tumours was delayed revealed that tumour cells were continuously dying for at least 24 hr after heat treatment. The volume of the tumour vasculature was considerably reduced after treatment, suggesting that the delayed cell death was attributed to vascular occlusion which resulted in an insufficient supply of oxygen and nutrients and an increased tumour acidity. The present work indicates that at least two mechanisms may be involved in heat-induced cell inactivation in our xenograft: firstly, direct cytotoxic effect of heat; secondly, indirect effect following heat-induced vascular damage.
研究了42.5摄氏度的热疗对无胸腺小鼠体内人黑色素瘤异种移植物的影响。肿瘤在水浴中进行体内加热。将肿瘤生长延迟和体外单细胞存活作为终点指标。通过微血管造影分析获得了关于热诱导血管损伤的定性信息。给定治疗后的肿瘤生长延迟比治疗后立即在体外测量的细胞存活所预期的要长得多。延迟切除肿瘤的实验表明，热处理后肿瘤细胞持续死亡至少24小时。治疗后肿瘤脉管系统的体积显著减小，这表明延迟的细胞死亡归因于血管闭塞，导致氧气和营养物质供应不足以及肿瘤酸度增加。目前的研究表明，在我们的异种移植物中，热诱导细胞失活可能至少涉及两种机制：第一，热的直接细胞毒性作用；第二，热诱导血管损伤后的间接作用。
