与小鼠骨髓瘤细胞在体内产生的C型颗粒相关的RNA指导的DNA聚合酶。

RNA-instructed DNA polymerase associated with C-type particles produced in vivo by murine myeloma cells.

作者信息

Yaniv A, Kleinman R, Eylan E

出版信息

J Gen Virol. 1976 Aug;32(2):301-9. doi: 10.1099/0022-1317-32-2-301.

Abstract

C-type particles secreted in vivo by MOPC-315 myeloma cells were characterized. These particles localize at a density of 1-16 g/ml in sucrose and possess a 60 to 70S RNA and an RNA-instructed DNA polymerase. Endogenous enzyme activity requires manganese and is inhibited by ribonuclease or by the omission of any of the deoxynucleoside triphosphates. The enzyme utilizes the virus 60 to 70S RNA as a template to synthesize DNA molecules which specifically hybridize to the homologous RNA.

摘要

对MOPC - 315骨髓瘤细胞在体内分泌的C型颗粒进行了表征。这些颗粒在蔗糖中的密度为1 - 16 g/ml，含有60至70S的RNA和一种RNA指导的DNA聚合酶。内源性酶活性需要锰，并且会被核糖核酸酶或任何一种脱氧核苷三磷酸的缺失所抑制。该酶利用病毒60至70S的RNA作为模板来合成与同源RNA特异性杂交的DNA分子。