鸡卵黄可稳定培养的MOPC - 315小鼠骨髓瘤细胞产生的C型颗粒中的逆转录酶活性。

Chicken egg yolk stabilizes the reverse transcriptase activity in type C particles produced by cultured MOPC-315 murine myeloma cells.

作者信息

Gazit A, Yaniv A, Eylan E

出版信息

Appl Environ Microbiol. 1978 Sep;36(3):397-402. doi: 10.1128/aem.36.3.397-402.1978.

DOI:10.1128/aem.36.3.397-402.1978

PMID:83122

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC243059/

Abstract

Type C particles released from cultured murine myeloma MOPC-315 cells were significantly protected when the purification steps were all conducted in the presence of 10% chicken egg yolk fluid. The yolk fluid also slowed down the inactivation of viral particles during incubation at 37 degrees C and enabled full recovery of viral particles through several cycles of freezing and thawing. The purification of viral particles in the presence of yolk fluid did not affect the capability of the viral DNA polymerase to reverse-transcribe the virion RNA in vitro, nor that of the viral RNA to act as a functional template.

摘要

当所有纯化步骤都在10%鸡卵黄液存在的情况下进行时，从培养的鼠骨髓瘤MOPC - 315细胞释放的C型颗粒受到显著保护。卵黄液还减缓了病毒颗粒在37摄氏度孵育期间的失活，并能通过几个冻融循环使病毒颗粒完全恢复。在卵黄液存在的情况下纯化病毒颗粒，并不影响病毒DNA聚合酶在体外逆转录病毒粒子RNA的能力，也不影响病毒RNA作为功能模板的能力。

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