Launay J M, Geoffroy C, Costa J L, Alouf J E
Thromb Res. 1984 Jan 15;33(2):189-96. doi: 10.1016/0049-3848(84)90179-8.
The purpose of this study was to examine the lytic effect on human platelet preparations (washed, gel-filtered and dextran-isolated) of two sulfhydryl-activated bacterial protein toxins, streptolysin 0 and and alveolysin, and to compare their efficacy with that of other disruptive procedures (freezing and thawing, ultrasonic, mechanical, or nystatin-toluene treatment) as a method for the determination of various platelet enzyme activities. The enzymes assayed were alkaline and acid phosphatases, monoamine oxidase, phenolsulfotransferase, N-acetyltransferase, hydroxyindole-O-methyltransferase, glutathione peroxidase and lactate dehydrogenase. In all cases, the lowest activities were found after freezing and thawing and/or ultrasonic disruption. The highest activities were always observed in the platelet lysates obtained after toxin, and in some instances after nystatin-toluene treatment. Intermediate values were obtained for mechanical disruption. The -SH-activated cytolysins thus appear to be appropriate and gentle tools for the assay of platelet enzymes when compared to the physical or chemical procedures generally employed.
本研究的目的是检测两种巯基激活的细菌蛋白毒素——链球菌溶血素O和肺泡溶血素对人血小板制剂（洗涤血小板、凝胶过滤血小板和葡聚糖分离血小板）的溶解作用，并将它们的效力与其他破坏方法（冻融、超声、机械或制霉菌素-甲苯处理）相比较，作为测定各种血小板酶活性的一种方法。所测定的酶有碱性磷酸酶、酸性磷酸酶、单胺氧化酶、酚磺基转移酶、N-乙酰转移酶、羟基吲哚-O-甲基转移酶、谷胱甘肽过氧化物酶和乳酸脱氢酶。在所有情况下，冻融和/或超声破碎后酶活性最低。毒素处理后获得的血小板裂解物中酶活性总是最高，在某些情况下，制霉菌素-甲苯处理后的酶活性也最高。机械破碎得到的酶活性值处于中间水平。因此，与通常采用的物理或化学方法相比，巯基激活的溶细胞素似乎是检测血小板酶的合适且温和的工具。
