硫醇激活的溶细胞素的疏水特性。
The hydrophobic character of thiol-activated cytolysins.
作者信息
Johnson M K, Knight R J, Drew G K
出版信息
Biochem J. 1982 Dec 1;207(3):557-60. doi: 10.1042/bj2070557.
Abstract
Hydrophobic chromatography on phenyl-Sepharose has revealed the decidedly hydrophobic character of several members of the group of cytolytic proteins termed ;thiol-activated'. Pneumolysin, alveolysin, cereolysin, and streptolysin O were found to be equally hydrophobic, as were the oxidized and reduced forms of alveolysin. Hydrophobic chromatography has been utilized in the development of an improved procedure for the purification of pneumolysin.
摘要
在苯基琼脂糖上进行的疏水层析显示，被称为“硫醇激活”的细胞溶解蛋白组中的几个成员具有明显的疏水特性。发现肺炎溶血素、肺泡溶血素、cereolysin和链球菌溶血素O具有同等的疏水性，肺泡溶血素的氧化形式和还原形式也是如此。疏水层析已被用于开发一种改进的肺炎溶血素纯化方法。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
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Infect Immun. 1980 Jan;27(1):97-101. doi: 10.1128/iai.27.1.97-101.1980.
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Properties of purified pneumococcal hemolysin.纯化肺炎球菌溶血素的特性
Infect Immun. 1972 Nov;6(5):755-60. doi: 10.1128/iai.6.5.755-760.1972.
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