Cornelissen B J, Brederode F T, Moormann R J, Bol J F
Nucleic Acids Res. 1983 Mar 11;11(5):1253-65. doi: 10.1093/nar/11.5.1253.
Double-stranded cDNA of alfalfa mosaic virus (AlMV) RNA 1 has been cloned and sequenced. From clones with overlapping inserts, and other sequence data, the complete primary sequence of the 3644 nucleotides of RNA 1 was deduced: a long open reading frame for a protein of Mr 125,685 is flanked by a 5'-terminal sequence of 100 nucleotides and a 3' noncoding region of 163 nucleotides, including the sequence of 145 nucleotides the three genomic RNAs of AlMV have in common. The two UGA-termination codons halfway RNA 1, that were postulated by Van Tol et al. (FEBS Lett. 118, 67-71, 1980) to account for partial translation of RNA 1 in vitro into Mr 58,000 and Mr 62,000 proteins, were not found in the reading frame of the Mr 125,685 protein.
苜蓿花叶病毒（AlMV）RNA 1的双链cDNA已被克隆并测序。根据具有重叠插入片段的克隆以及其他序列数据，推导得出RNA 1的3644个核苷酸的完整一级序列：一个编码Mr 125,685蛋白质的长开放阅读框两侧分别是100个核苷酸的5'末端序列和163个核苷酸的3'非编码区，包括AlMV三种基因组RNA共有的145个核苷酸序列。Van Tol等人（《欧洲生物化学学会联合会快报》118，67 - 71，1980）推测RNA 1中间的两个UGA终止密码子可解释RNA 1在体外部分翻译为Mr 58,000和Mr 62,000蛋白质的现象，但在Mr 125,685蛋白质的阅读框中未发现这两个终止密码子。
