Kuhn N J, Woodworth-Gutai M, Gross K W, Held W A
Nucleic Acids Res. 1984 Aug 10;12(15):6073-90. doi: 10.1093/nar/12.15.6073.
The mouse major urinary proteins (MUPs) are the products of a multi-gene family of 30-35 genes whose members exhibit diverse tissue specific, developmental, and hormonal controls. Three cDNA clones corresponding to liver MUP mRNAs have been sequenced. Two of the clones (p499, C57BL/6 and p1057, BALB/c) share strong homology whereas a third clone (p199, C57BL/6) has diverged considerably from the others at the nucleic acid (85% homology) and protein (68% homology) levels. The 5' regions of p499 and p199 which show the most sequence divergence were subcloned and shown to hybridize to different liver MUP mRNAs. The p499-5' sequence was expressed in all MUP expressing tissues (liver, lachrymal, submaxillary and mammary) whereas the p199-5' sequence was expressed primarily in the liver and lachrymal. Analysis of liver RNA from mice in different endocrine states indicates that the p499-5' sequence is strongly regulated by thyroxine administration whereas the p199-5' sequence is not. Both sequences appear to be regulated by growth hormone and testosterone. Southern blot analysis of mouse genomic DNA indicates that there are multiple genes homologous to each sequence.
小鼠主要尿蛋白（MUPs）是一个由30 - 35个基因组成的多基因家族的产物，其成员表现出不同的组织特异性、发育调控和激素调控。已对三个与肝脏MUP mRNA相对应的cDNA克隆进行了测序。其中两个克隆（p499，C57BL/6和p1057，BALB/c）具有很强的同源性，而第三个克隆（p199，C57BL/6）在核酸（85%同源性）和蛋白质（68%同源性）水平上与其他克隆有很大差异。对p499和p199的5'区域进行亚克隆，这两个区域序列差异最大，结果显示它们与不同的肝脏MUP mRNA杂交。p499 - 5'序列在所有表达MUP的组织（肝脏、泪腺、颌下腺和乳腺）中都有表达，而p199 - 5'序列主要在肝脏和泪腺中表达。对处于不同内分泌状态的小鼠肝脏RNA进行分析表明，p499 - 5'序列受甲状腺素给药的强烈调控，而p199 - 5'序列则不受其调控。这两个序列似乎都受生长激素和睾酮的调控。对小鼠基因组DNA进行Southern印迹分析表明，与每个序列同源的基因有多个。
