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编码小鼠主要尿蛋白的功能基因的鉴定与特性分析。

Identification and characterization of functional genes encoding the mouse major urinary proteins.

作者信息

Held W A, Gallagher J F, Hohman C M, Kuhn N J, Sampsell B M, Hughes R G

机构信息

Department of Molecular and Cellular Biology, Roswell Park Memorial Institute, Buffalo, New York 14263.

出版信息

Mol Cell Biol. 1987 Oct;7(10):3705-12. doi: 10.1128/mcb.7.10.3705-3712.1987.

DOI:10.1128/mcb.7.10.3705-3712.1987
PMID:2824995
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC368026/
Abstract

Mouse Ltk- cells were stably transfected with cloned genes encoding the mouse major urinary proteins (MUPs). C57BL/6J MUP genomic clones encoding MUP 2 (BL6-25 and BL6-51), MUP 3 (BL6-11 and BL6-3), and MUP 4 (BL6-42) have been identified. In C57BL/6J mice, MUP 2 and MUP 4 are known to be synthesized in male, but not female, liver, and MUP 3 is known to be synthesized in both male and female liver and mammary gland. A BALB/c genomic clone (BJ-31) was shown to encode a MUP that is slightly more basic than MUP 2 and was previously shown to be synthesized in both male and female liver of BALB/c but not C57BL/6 mice. Comigration on two-dimensional polyacrylamide gels of the MUPs encoded by the transfecting gene provides a basis for tentative identification of the tissue specificity and mode of regulation of each gene. DNA sequence analysis of the 5' flanking region indicates that the different MUP genes are highly homologous (0.20 to 2.40% divergence) within the 879 base pairs analyzed. The most prominent differences in sequence occur within an A-rich region just 5' of the TATA box. This region (from -47 to -93) contains primarily A or C(A)N nucleotides and varies from 15 to 46 nucleotides in length in the different clones.

摘要

将编码小鼠主要尿蛋白（MUPs）的克隆基因稳定转染至小鼠Ltk-细胞。已鉴定出编码MUP 2（BL6 - 25和BL6 - 51）、MUP 3（BL6 - 11和BL6 - 3）和MUP 4（BL6 - 42）的C57BL/6J MUP基因组克隆。在C57BL/6J小鼠中，已知MUP 2和MUP 4在雄性而非雌性肝脏中合成，且已知MUP 3在雄性和雌性肝脏及乳腺中均有合成。一个BALB/c基因组克隆（BJ - 31）被证明编码一种比MUP 2碱性略强的MUP，且先前已证明其在BALB/c的雄性和雌性肝脏中均有合成，但在C57BL/6小鼠中无合成。转染基因编码的MUP在二维聚丙烯酰胺凝胶上的共迁移为初步鉴定每个基因的组织特异性和调控模式提供了基础。对5'侧翼区域的DNA序列分析表明，在所分析的879个碱基对内，不同的MUP基因高度同源（差异为0.20%至2.40%）。序列中最显著的差异出现在TATA框上游仅5'处的富含A的区域。该区域（从 - 47至 - 93）主要包含A或C(A)N核苷酸，且在不同克隆中的长度从15至46个核苷酸不等。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f8/368026/efb7bdc1ab3e/molcellb00082-0348-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f8/368026/2bf60f676d3a/molcellb00082-0347-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f8/368026/091b2e390509/molcellb00082-0347-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f8/368026/e2f471becc36/molcellb00082-0347-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f8/368026/efb7bdc1ab3e/molcellb00082-0348-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f8/368026/2bf60f676d3a/molcellb00082-0347-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f8/368026/091b2e390509/molcellb00082-0347-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f8/368026/e2f471becc36/molcellb00082-0347-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f8/368026/efb7bdc1ab3e/molcellb00082-0348-a.jpg

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Mol Cell Biol. 1987 Oct;7(10):3705-12. doi: 10.1128/mcb.7.10.3705-3712.1987.
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引用本文的文献

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2
Nonsense but not missense mutations can decrease the abundance of nuclear mRNA for the mouse major urinary protein, while both types of mutations can facilitate exon skipping.无义突变而非错义突变可降低小鼠主要尿蛋白的核mRNA丰度，而这两种类型的突变均可促进外显子跳跃。
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本文引用的文献

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ELECTROPHORETIC VARIATION AND SEX DIMORPHISM OF THE MAJOR URINARY PROTEIN COMPLEX IN INBRED MICE: A NEW GENETIC MARKER.近交系小鼠主要尿蛋白复合物的电泳变异与性别二态性：一种新的遗传标记
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In vitro synthesis and characterization of precursors to the mouse major urinary proteins.小鼠主要尿蛋白前体的体外合成与表征
J Biol Chem. 1980 Feb 25;255(4):1367-73.
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Structural genes of the mouse major urinary protein are on chromosome 4.小鼠主要尿蛋白的结构基因位于4号染色体上。
A Mup promoter-thymidine kinase reporter gene shows relaxed tissue-specific expression and confers male sterility upon transgenic mice.
一个Mup启动子-胸苷激酶报告基因表现出宽松的组织特异性表达，并使转基因小鼠产生雄性不育。
Mol Cell Biol. 1988 Nov;8(11):4821-8. doi: 10.1128/mcb.8.11.4821-4828.1988.
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Subfamily of submaxillary gland-specific Mup genes: chromosomal linkage and sequence comparison with liver-specific Mup genes.下颌下腺特异性Mup基因亚家族：染色体连锁及与肝脏特异性Mup基因的序列比较
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Silent genes in the mouse major urinary protein gene family.小鼠主要尿蛋白基因家族中的沉默基因。
Proc Natl Acad Sci U S A. 1989 Jun;86(12):4584-8. doi: 10.1073/pnas.86.12.4584.
7
T antigen expression and tumorigenesis in transgenic mice containing a mouse major urinary protein/SV40 T antigen hybrid gene.含有小鼠主要尿蛋白/SV40 T抗原杂交基因的转基因小鼠中的T抗原表达与肿瘤发生
EMBO J. 1989 Jan;8(1):183-91. doi: 10.1002/j.1460-2075.1989.tb03363.x.
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Intraspecific evolution of a gene family coding for urinary proteins.
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Hepatocyte-stimulating factor, beta 2 interferon, and interleukin-1 enhance expression of the rat alpha 1-acid glycoprotein gene via a distal upstream regulatory region.
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The cytokine response element of the rat alpha 1-acid glycoprotein gene is a complex of several interacting regulatory sequences.大鼠α1-酸性糖蛋白基因的细胞因子反应元件是由几个相互作用的调控序列组成的复合体。
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Differential, multihormonal regulation of the mouse major urinary protein gene family in the liver.小鼠肝脏中主要尿蛋白基因家族的差异多激素调控。
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The locus of sequence-directed and protein-induced DNA bending.序列导向和蛋白质诱导的DNA弯曲位点。
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